Effect of Feeding Thymolina Powder on the Immune System and Gene Expression Interleukin II

Seyedmousa Hosseini; Mohammad  Chamani; Alireza   Seidavi; Ali Asghar  Sadeghi; Zarbakht  Ansari-Pirsaraei

Seyedmousa Hosseini Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran https://orcid.org/0000-0002-4371-0403
Mohammad Chamani Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Alireza Seidavi Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Ali Asghar Sadeghi Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Zarbakht Ansari-Pirsaraei Department of Animal Science, Faculty of Animal and Fishery Sciences, Agricultural Science and Natural Resources University of Sari, Sari, Iran

Keywords: Immune, Interleukin II, Gene Expression, Thymolina, Ross 308, Broiler

Abstract

Medicinal plants are beneficial to intestinal palatability and performance, have antimicrobial properties, have a wide range of antioxidant activities, stimulate the immune system, and promote nutritional absorption. 320 one-day-old Ross 308 broiler chicks were utilised in this study, which followed a completely randomised design with four treatments, four replicates, and each replicate contained 20 broiler chickens. Control, 0.5, 1, and 2% in the food of hens are among the experimental treatments. Thymolina is an antibacterial powder medication derived from the combination of four medicinal herbs (Salvia officinalis, Matricaria chamomilla, Teucrium polium and Origanum majorana). During the experiment period, chickens had free access to water and food and the livestock raising management was conducted in terms of lighting, humidity, ventilation, and vaccination in accordance with the guide requirements of Ross 308 broiler chickens. Weighting was conducted weekly and at the end of feed intake, weight gain and feed conversion ratio of chickens were measured. To investigate the body’s immune system of broiler chickens, the HI and SRBC tests were used. To do so, each replicate of a bird was selected and 1 ml per kg of live weight of chickens of sheep red blood cells (SRBC) was injected was injected into the pectoral muscle at the age of 28 years old. 7 days after the injection, blood sampling from the brachial vein of the same birds as 2 ml was conducted and blood serum was separated by centrifugation at 3000g for 10 min. the amount of antibody titers against SRBC in serum obtained using the HA method and concentration of Newcastle disease vaccine titers obtained by HI were determined. In addition, to the gene expression of interleukin II, the same birds were selected, and at the end of the period, the amount of 1-2 ml blood was taken under the wing of the venous vessel, and then the investigation of the amount of the gene expression of interleukin II was conducted using specific primers, GEN, and Real-Time PCR Reaction. Newcastle disease vaccine titers and SRBC were experimented with by experimental treatments and the alloy of anti-body titers increased. The gene expression of interleukin II increased and significant differences among different treatments were observed (P<0.05). Overall, the results showed that including Thymolina in the diet of broiler chickens improves their immune system by raising IL-2 gene expression. Therefore, it can be effective on the immune responses of broiler chickens and cause the improvement creation of the favorable and effective immune system in broiler chickens.

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